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运用荧光标记的抗细胞表面抗原单抗及抗细胞因子单抗， 结合固定、破膜处理技术， 建立了三荧光染色法流式细胞术检测细胞内细胞因子的方法， 探讨了不同刺激剂及细胞培养时间的选择， 并对５ 例正常人产生ＩＦＮγ、ＩＬ４ 的ＣＤ４ 及ＣＤ８ 细胞进行测定。结果表明： 产生ＩＦＮγ的ＣＤ４ 及ＣＤ８ 细胞明显多于产生ＩＬ４ 的细胞， 并且产生ＩＦＮγ的ＣＤ８ 细胞百分数比较ＣＤ４ 细胞相对增多； 同时产生ＩＦＮγ／ＩＬ４ 的ＣＤ４ 、ＣＤ８ 细胞较少。此方法可从单个细胞水平直接用于Ｔ细胞亚群及其细胞因子的检测。
Using fluorescent-labeled anti-cell surface antigen monoclonal antibody and anti-cytokine monoclonal antibody, combined with fixed, rupture membrane technology, the establishment of a three-fluorescence staining flow cytometry intracellular cytokines, explore the different stimulators and cells The choice of culture time, and 5 cases of normal human IFN γ, IL 4 CD4 and CD8 cells were measured. The results showed that: IFN γ-producing CD4 and CD8 cells were significantly more than those producing IL 4 cells, and the percentage of CD8 cells producing IFN γ relative CD4 cells increased; also produce IFN γ / IL 4 CD4, CD8 Less cells. This method can be used directly at the individual cell level for the detection of T-cell subsets and their cytokines.