方法:以2个注射器分别采静脉血0.5毫升,1个注射器内有依地酸(EDTA)缓冲液-甲醛液2毫升,另一个仅含依地酸缓冲液2毫升。(前者配方: 3毫升0.077克分子依地酸,5毫升4％甲醛液,2毫升10倍浓度之磷酸缀冲盐溶液,10毫升蒸馏水。后者配方:3毫升0.077克分子依地酸,5毫升10倍浓度之磷酸缓冲盐溶液,12毫升蒸馏水。二者均为pH7.4的等渗溶液)。充分混匀后装入涂有硅油的试管 Methods: The two syringes were collected venous blood 0.5 ml, 1 syringe with EDTA solution - formaldehyde solution 2 ml, the other containing only 2 ml edetate buffer. (The former formula: 3 ml of 0.077 g of molecular acid, 5 ml of 4% formalin, 2 ml of 10 times concentration of phosphoric acid salt-coated salt solution, 10 ml of distilled water.The latter formula: 3 ml 0.077 g molecular acid, 5 Ml 10-fold concentration of phosphate buffered saline, 12 ml of distilled water, both at an isotonic pH of 7.4). Mix well and load into a test tube coated with silicone oil