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目的认识不同表面分子标记物所标记的脾脏树突状细胞(dendritic cell,DC)分布、成熟程度与超微结构特点。方法运用光镜、电镜观察与免疫组化标记(CD11c、CD205、CD209、S-100、CD1a、HLA-DR、CD80及CD86)方法观察35例正常人脾脏组织中的树突状细胞的形态分布及超微结构特点。结果正常人脾脏树突状细胞主要分布在白髓边缘区和T细胞区,CD11c、CD205和CD209是目前较特异的树突状细胞标志物,标记阳性率分别为4.19%、3.96%和3.02%,其中CD209+ DC分布更靠近红髓。树突状细胞表达共刺激分子CD80与CD86的阳性率分别为2.68%和2.02%,主要分布在T细胞区。树突状细胞超微结构特点为细胞体积较大,呈细长状或不规则形,表面可见数个突起向周围细胞间隙延伸;胞核形状不规则,表面有凹陷,可见核仁;胞浆致密或呈低电子密度,细胞器欠发达。结论正常人脾脏中不同表面分子阳性树突状细胞的比率和分布有所不同,其中大部分为不成熟的树突状细胞,具有活性的成熟树突状细胞数目较少。
Objective To understand the distribution, maturity and ultrastructure of dendritic cells (DCs) labeled by different surface markers. Methods The morphological distribution of dendritic cells in 35 normal human spleens was observed by light microscopy, electron microscopy and immunohistochemical staining (CD11c, CD205, CD209, S-100, CD1a, HLA-DR, CD80 and CD86) And ultrastructural features. Results Normal human spleen dendritic cells mainly distributed in marginal zone of white pulp and T cell area. CD11c, CD205 and CD209 were the specific dendritic cells markers with the positive rates of 4.19%, 3.96% and 3.02% , Of which CD209 + DC distribution closer to the red pulp. The positive rates of CD80 and CD86 expression of dendritic cells were 2.68% and 2.02%, respectively, which were mainly distributed in T cells. The ultrastructure of dendritic cells is characterized by large cell volume, slender or irregular shape, the surface of the number of protrusions can be seen extending to the surrounding cell gap; irregular nuclear shape, surface depression, visible nucleolus; cytoplasm Dense or low electron density, less developed organelles. Conclusion The percentage and distribution of dendritic cells with different surface molecules in spleen of normal people are different. Most of them are immature dendritic cells, and the number of active dendritic cells is less.