暗视野显微镜在生物学教学和科研中的用途是十分广泛的。我们在教学中,应用普通光学显微镜改成暗视野显微镜,改装后的效果与暗视野显微镜几乎相同。具体改制方法如下。将明视野显微镜按正常操作程序进行调节,使视野明亮。取下目镜,通过镜筒观看视野,同时调节光圈,使之与视野同样大小。倾斜镜臂,用圆规或尺子在聚光器下量出光圈直径的大小。用一块黑色纸做一个与光圈直径相同或稍大一些的圆形挡光片,贴在滤光镜片中央,置于滤光镜片支架上。通过镜筒观看视野,并调节黑色 Dark-field microscope in biology teaching and research purposes is very wide. We in teaching, the application of ordinary optical microscope into a dark field microscope, the modified effect and dark field microscope is almost the same. The specific restructuring method is as follows. The bright field microscope according to the normal operating procedures to adjust, so bright field of vision. Remove the eyepiece and view the field of view through the lens barrel while adjusting the aperture so that it is the same size as the field of view. Tilt the mirror arm and use a compass or ruler to measure the diameter of the diaphragm under the condenser. Use a piece of black paper to make a circular light-blocking film with the same or slightly larger diameter as the aperture, affixed to the middle of the filter and placed on the filter holder. View the field of view through the lens barrel, and adjust the black