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目的分析TB-IGRA定量试验对肺结核病的诊断价值。方法收集患者全血及晨痰标本,分别采用TBIGRA定量法、胶体金法和FQ-PCR法检测结核特异性γ-干扰素的含量、结核抗体、痰中结核分枝杆菌核酸。结果 TB-IGRA的特异性为92.45%,敏感性为91.02%;78例肺结核病患者中,结核分枝杆菌DNA、痰抗酸染色涂片镜检、结核抗体检测的阳性率依次为76.92%、67.95%、43.59%,经χ~2检验,TB-IGRA与其他3种检测方法阳性率差异有统计学意义(P<0.01,P<0.05,P<0.01)。结论 TB-IGRA具有较好的特异性和灵敏性,有利于肺结核病的及时、准确诊断。
Objective To analyze the diagnostic value of TB-IGRA quantitative test for pulmonary tuberculosis. Methods Whole blood and sputum samples were collected. TBIGRA quantitative assay, colloidal gold assay and FQ-PCR assay were used to detect tuberculosis-specific IFN-γ, tuberculosis antibodies and Mycobacterium tuberculosis in sputum. Results The specificity of TB-IGRA was 92.45% and the sensitivity was 91.02%. The positive rates of TB and IGT in 78 cases of pulmonary tuberculosis were 76.92% 67.95% and 43.59% respectively. There was significant difference between the two groups (P <0.01, P <0.05, P <0.01) byχ2 test and TB-IGRA. Conclusion TB-IGRA has good specificity and sensitivity, which is helpful for the timely and accurate diagnosis of pulmonary tuberculosis.