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目的:考察积雪草苷立方液晶的制备工艺及含量测定方法。方法:前体-注入法制备积雪草苷立方液晶,用均匀设计筛选优化制备工艺,透析法分离游离药物,结合HPLC法,流动相:乙腈:2 mmol/Lβ-环糊精(24∶76),柱温25℃,检测波长205 nm,流速0.8 mL/min,进样量10μL,在此色谱条件下测积雪草苷包封率和载药量。结果:透析时间12 h,游离药物基本从积雪草苷立方液晶中分离,经均匀设计试验验证积雪草苷立方液晶的药物包封率可达39.8%。结论:前体-注入法制备积雪草苷立方液晶包封率高、简便可行,透析法结合HPLC测定药物含量准确。
Objective: To investigate the preparation technology and determination of asiaticoside cubic liquid crystal. METHODS: Asiaticoside cubic liquid crystal was prepared by the precursor-injection method. The optimal preparation process was determined by uniform design and screening. The free drug was separated by dialysis method. The mobile phase consisted of acetonitrile: 2 mmol / L β-cyclodextrin ), The column temperature was 25 ℃, the detection wavelength was 205 nm, the flow rate was 0.8 mL / min and the injection volume was 10 μL. The encapsulation efficiency and drug loading of asiaticoside were measured under this chromatographic conditions. Results: The dialysis time was 12 h. The free drug was separated from asiaticoside cubic liquid crystal. The uniform encapsulation efficiency of asiaticoside cubic liquid crystal was 39.8%. Conclusion: The precursor - injection method for the preparation of asiaticoside cubic liquid crystal encapsulation efficiency is simple and feasible dialysis method combined with HPLC determination of drug content is accurate.