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目的:本研究通过糖尿病大鼠结肠生物力学重构与糖基化终末产物表达的相关性来探讨肠润通的干预作用机制。方法:将成年健康SD大鼠40只,每组10只随机分为空白对照组,糖尿病对照组,肠润通高(50g/kg)、低(25g/kg)剂量治疗组。肠润通每日1次经灌胃给药,定期检测血糖和体重,实验周期为60d。实验结束时,通过免疫组织化学染色方法观测各组糖基化终末产物(AGEs)及其受体(RAGE)在结肠肠壁各层的分布表达,利用图像分析软件对其表达进行定量分析。结肠管壁的湿重,壁厚、展开角、残余应变以及应力应变等形态学和生物力学参数取自本课题组之前相关研究报道的结果。采用线性回归方法对上述两组参数进行相关统计分析。结果:糖尿病对照组AGEs和RAGE在结肠肠壁各层的表达明显高于空白对照组(P<0.05,P<0.01)且与形态组织学和生物力学重构参数高度正相关。经肠润通治疗后,高剂量肠润通处理能显著降低AGEs和RAGE在结肠肠壁各层的表达(P<0.05,P<0.01),但低剂量肠润通处理无显著作用。结论:AGEs和RAGE在肠壁各层组织的表达与糖尿病结肠生物力学特性重构高度相关,高剂量肠润通对AGEs-RAGE表达的抑制作用是糖尿病结肠生物力学特性重构机制改善的有效途径之一。
OBJECTIVE: To investigate the mechanism of intervention of Changrun Tong through the correlation between the biomechanical remodeling of colon and the expression of advanced glycation end products in diabetic rats. Methods: Forty adult healthy SD rats were randomly divided into blank control group, diabetic control group, Changrongtonghuan (50g / kg) and low (25g / kg) treatment groups. Intestinal Run through once daily gavage, regular blood sugar and body weight testing, the experimental period of 60d. At the end of the experiment, the expression and distribution of AGEs and its receptor (RAGE) were observed by immunohistochemical staining in each layer of the colon wall. The expression of AGEs and its receptor (RAGE) was quantitatively analyzed by image analysis software. Morphology and biomechanical parameters such as wet weight, wall thickness, unfolding angle, residual strain and stress-strain of the colon wall were taken from the results of previous studies reported by our group. Linear regression method was used to analyze the above two parameters. Results: The expressions of AGEs and RAGE in diabetic control group were significantly higher than those in blank control group (P <0.05, P <0.01), and were positively correlated with morphological and biomechanical parameters. After treatment with enteral nutrition, high-dose enteral nutrition can significantly reduce the expression of AGEs and RAGE in all layers of colon (P <0.05, P <0.01), but no effect of low-dose enteral nutrition. CONCLUSION: The expression of AGEs and RAGE in the layers of the intestinal wall is highly correlated with the biomechanical properties of diabetic colon. The inhibition of AGEs-RAGE expression by high-dose gut Run Tong is an effective way to improve the mechanism of biomechanics of diabetic colon one.