目的 :自行合成LewisY拟糖脂并寻找一种合适的标记拟糖脂的方法。方法 :应用LewisY四糖合成了LewisY拟糖脂 ,以此制备了含3H 胆固醇标记和LewisY拟糖脂的脂质体 ,利用LewisY糖特异性的抗体鉴定了糖脂的免疫学活性 ,并检测了LewisY拟糖脂 脂质体与该抗体的结合能力。结果 :在适宜条件下 ,LewisY四糖拟糖脂的合成率为 4 0 % ,合成的特异性糖脂高效薄层层析为单一条带 ;制备的LewisY拟糖脂 脂质体与Lewis寡糖抗体具有特异结合能力。结论 :以3H 胆固醇标记的LewisY拟糖脂 脂质体是一种效果良好的间接标记的拟糖脂探针。 OBJECTIVE: To synthesize Lewis-derived glycolipid by itself and find a suitable method for labeling the proposed glycolipid. Methods: LewisY tetrasaccharide was used to synthesize LewisY-linked glycolipid. Liposomes containing 3H-cholesterol labeled and LewisY-linked glycolipid were prepared. The immunological activity of glycolipids was identified by LewisY-specific antibody. LewisY-derived glycolipid liposomes with the ability of the antibody. Results: Under appropriate conditions, the synthesis rate of Lewis Y tetrasaccharide glycolipid was 40%, and the synthesized specific glycolipid was a single band by high performance thin layer chromatography. The prepared Lewis Y antisaccharide liposomes and Lewis oligosaccharide Antibodies have specific binding capacity. CONCLUSION: The Lewis cholesterol glycolipid labeled with 3H-cholesterol is a good indirect marker of MIPs.