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对33只大鼠行腹主动脉缩窄10天,建立心肌肥大动物模型。将左心室前壁中份心内膜下层和心外膜下层心肌组织,放入含1%硝酸镧的2%戊二醛二甲砷酸钠缓冲液(pH 7.4)3小时。结果发现,对照组动物的心外膜下层和心内膜下层心肌的超微结构正常,镧沉淀局限于细胞外间隙、横小管和闰盘,并沉着于基膜。在实验组,心内膜下层心肌超微结构改变明显,镧进入细胞内,线粒体膜上出现特征性的镧沉淀;心外膜下层心肌超微结构改变不明显,但多数心肌细胞内可见镧沉淀。结果表明,肥大心肌细胞膜通透性改变,可能是心肌细胞损害的早期特征之一,镧可以作为显示肥大心肌细胞膜通透性变化的敏感的示踪物。
Thirty-three rats were subjected to abdominal aorta constriction for 10 days to establish an animal model of cardiac hypertrophy. The middle part of the anterior wall of the left ventricle and the subepicardial myocardium were placed in a 2% glutaraldehyde sodium cacodylate buffer (pH 7.4) containing 1% lanthanum nitrate for 3 hours. The results showed that the ultrastructure of the epicardial and subendocardial myocardium in the control animals was normal. The lanthanum precipitation was confined to the extracellular space, the lateral tubules and the intercalated disc and settled on the basilar membrane. In the experimental group, ultrastructural changes of the subendocardial myocardium were obvious. Lanthanum entered the cells, and lanthanum was found on the mitochondria membrane. The ultrastructure of the epicardial myocardium did not change obviously. However, most of the myocardial cells showed lanthanum precipitation . The results show that hypertrophic cardiomyocyte membrane permeability changes may be one of the early characteristics of myocardial cell damage, lanthanum can be used as a sensitive tracer to show changes in membrane permeability of hypertrophic cardiomyocytes.