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目的探讨薯蓣皂苷对胶原诱导型关节炎小鼠的治疗作用及其机制。方法选用DBA1/J小鼠建立鸡Ⅱ型胶原诱导型关节炎小鼠模型(CIA),将造模成功的小鼠采用随机数字表法分为CIA模型组、薯蓣皂苷组、雷公藤多苷组,另设正常对照组,药物组于初次免疫后第21天开始灌胃给药,对照组和模型组给相同体积溶媒。初次免疫第35天处死小鼠,无菌取腹股沟淋巴结制成单细胞悬液,用实时荧光定量PCR技术检测Th17、Treg细胞特异性转录因子RORγt、Foxp3的表达情况。结果模型组与对照组相比,RORγt显著升高(P<0.05);2药物组与模型组相比RORγt显著下降(P<0.05);与模型组相比,薯蓣皂苷组Foxp3显著升高(P<0.05)。结论薯蓣皂苷可以调节Th17、Treg细胞特异转录因子RORγt、Foxp3的平衡。
Objective To investigate the therapeutic effect of diosgenin on collagen-induced arthritis in mice and its mechanism. Methods DBA1 / J mice were used to establish collagen type II induced arthritis mouse model (CIA). The successful mice were randomly divided into CIA model group, diosgenin group, tripterygium glycosides group , Another normal control group, the drug group began on the 21st post-primary immunization gavage, the control group and model group to the same volume of vehicle. Mice were sacrificed on the 35th day of primary immunization, and the inguinal lymph nodes were aseptically removed to make single cell suspension. The expression of Th17 and Treg cell specific transcription factors RORγt and Foxp3 were detected by real-time fluorescence quantitative PCR. Results Compared with the control group, the RORγt of model group was significantly increased (P <0.05). Compared with the model group, the RORγt of the model group was significantly decreased (P <0.05). Compared with the model group, the Foxp3 of diosgenin group was significantly increased P <0.05). Conclusion Diosgenin can regulate the balance of Th17 and Treg cell-specific transcription factors RORγt and Foxp3.