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用固相化甲肝抗原，对所构建的噬菌体抗体库进行了３轮淘筛。第３轮淘筛后洗脱下来的噬菌体，较第１轮增加了近１００倍。含有抗体重链基因和轻链基因的重组克隆，也由淘筛前的２５％增至１００％。说明甲肝抗原对抗体库的淘筛，富集了表面呈现甲肝人单抗的噬菌体。经夹心ＥＬＩＳＡ法筛选到抗甲肝病毒噬菌体抗体，并以竞争抑制实验进一步证实了这些抗体的特异性。
The constructed phage antibody library was subjected to 3 rounds of panning using immobilized hepatitis A antigen. The third round of phage eluted after scouring, compared with the first round increased by nearly 100 times. Recombinant clones containing antibody heavy chain and light chain genes also increased from 25% before panning to 100%. Explain hepatitis A antigen on the antibody screening pan Amoy, enrichment of the surface of hepatitis A human monoclonal antibody phage. Anti-hepatitis A virus phage antibodies were screened by sandwich ELISA and the specificity of these antibodies was further confirmed by competition inhibition experiments.