一、活性物质的分离紫苏叶甲醇提取物口腔给药,(2g/kg),导致用六巴比妥处理的小鼠睡眠时间明显延长(84％,P<0.01)。为分离活性成分,提取物继续分成几个部分,每部分剂量按上部分与其本身被计算出的重量比,在小鼠身上进行活性效果实验。将甲醇提取物在CHCl_3(2.5L)和H_2O(0.51)两相间萃取,镇痛活性成分移至氯仿萃取液中,继之,通过硅胶柱(Wakogel— I. Separation of Active Substances Oral administration of the methanol extract of Perilla leaves (2 g/kg) resulted in a significant increase in sleep time in mice treated with hexabarbitol (84%, P < 0.01). In order to separate the active ingredients, the extract is further divided into several parts, and each part of the dose is subjected to an activity effect test on the mouse according to the weight ratio of the upper part to itself. The methanol extract was extracted between two phases of CHCl 3 (2.5L) and H 2 O (0.51), and the analgesic active ingredient was transferred to a chloroform extract followed by passing through a silica gel column (Wakogel—