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目的探讨直接测定兽肉中硝基呋喃类药物残留的方法。方法直接用酶联免疫吸附试验(ELISA)检测兽肉中硝基呋喃类中的呋喃西林代谢物氨基脲(SEM),以反映药物的残留情况。结果方法回收率在81.0%～90.0%之间,相对标准偏差为13.2%;该方法与高效液相色谱法比较,结果差异无统计学意义(P>0.05)。结论用ELISA法直接测定兽肉中呋喃西林代谢物SEM具有准确、简单和快速的优点。
Objective To explore the method of direct determination of nitrofuran residues in animal meat. Methods The nitrofuran metabolites semicarbazide (SEM) of nitrofurans in bestil meat was directly detected by enzyme-linked immunosorbent assay (ELISA) to reflect the drug residues. Results The recovery rate was between 81.0% and 90.0%, and the relative standard deviation was 13.2%. There was no significant difference between the method and HPLC (P> 0.05). Conclusion Direct determination of nitrofurantoin metabolites in veterinary meat by ELISA has the advantages of accuracy, simplicity and rapidity.