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以枣果实为试材,采用同源克隆的方法,利用转录组测序结果设计F3 H基因特异引物序列,成功克隆得到了枣果实F3 H基因全长,对其进行了生物信息学分析,并对该基因含量变化与花色苷的关系进行了研究。结果表明:F3 H基因全长1 336bp,编码367个氨基酸残基。根据基因序列比对结果,枣果实F3 H基因编码的氨基酸序列与龙眼(Dimocarpus longan)、橄榄(Canarium album)、荔枝(Litchi chinensis)及川桑(Morus notabilis)的一致性分别为90%、90%、89%、89%。半定量RT-PCR法分析表明,该基因在枣果实的红熟期表达量最大,其次为枣果实的半红期,而枣果实的绿熟期表达量最低;该基因表达量变化与花色苷含量变化一致。
Taking the jujube fruit as test material, the homologous cloning method was used to design the F3 H gene specific primer sequence by transcriptome sequencing. The full length of F3 H gene was successfully cloned and analyzed by bioinformatics method. The relationship between the changes of the gene content and anthocyanins was studied. The results showed that the F3 H gene was 1 336 bp in length and encoded 367 amino acid residues. According to the results of gene sequence alignment, the amino acid sequences of the F3 H gene of jujube fruit were 90%, 90%, 90% identical to Dimocarpus longan, Canarium album, Litchi chinensis and Morus notabilis respectively, , 89%, 89%. Semi-quantitative RT-PCR analysis showed that the expression of this gene was the highest at the red ripening stage of jujube fruit, followed by the half reddish jujube fruit, while the jujube fruit had the lowest green ripening stage; Content changes consistent.