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用密度梯度区带超速离心法分离了牛血浆高密度脂蛋白(HDL),经脱脂和凝胶层析(Sephadex G-200),得到载脂蛋白A-1(apo A-1)。SDS-PAGE电泳鉴定apoA-1的分子量为25.8kD。在apo A-1中不含有半胱氨酸,极性氨基酸多达33.6％。DABITC微量分析法实验结果证明apo A-1氨基酸末端是天门冬氨酸,与北京鸭apo A-1氨基末端相同。
Bovine plasma high density lipoprotein (HDL) was separated by density gradient zone ultracentrifugation and apolipoprotein A-1 (apo A-1) was obtained by defatting and gel filtration (Sephadex G-200). SDS-PAGE electrophoresis identified apoA-1 molecular weight of 25.8kD. Cysteine is absent in apo A-1 with up to 33.6% of polar amino acids. The results of DABITC microanalysis showed that the amino acid end of apo A-1 is aspartic acid, which is the same as that of Beijing duck apo A-1.