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目的观察清热涤痰方对垂体后叶素诱发高脂血症小鼠冠状动脉痉挛的影响,并探讨其可能的作用机制。方法将140只C57BL/6J小鼠随机分为正常组、模型组、合心爽组、阿托伐他汀组和中药高、中、低剂量组,每组20只。除正常组外,其余各组均给予高脂饲料饲养4周。中药高、中、低剂量组分别给予清热涤痰解痉方颗粒剂7.2、3.6、1.8 g/(kg·d),合心爽组给予合心爽0.04 g/(kg·d),阿托伐汀组给予阿托伐他汀钙片0.013 g/(kg·d),正常组和模型组予同体积0.5%羧甲基纤维素钠溶液,每日1次,连续4周。末次给药后1 h,腹腔注射垂体后叶素注射液60 U/kg建立冠状动脉痉挛模型。记录注射前10 min和注射后5、10、15 min时点Ⅱ导联心电图,计算T波位移值,试验结束后检测血清氧化型低密度脂蛋白(ox-LDL)、高敏C反应蛋白(hs-CRP)、内皮素1(ET-1)水平。结果模型组腹腔注射垂体后叶素后5、10、15 min时,小鼠心电图T波位移值较正常组有明显升高(P<0.05);与模型组比较,合心爽组、阿托伐他汀组和中药高、中、低剂量组在5、10、15 min时均能降低T波位移值(P<0.05);与阿托伐他汀组比较,中药高、中剂量组在5、10 min时T波位移值显著降低,中药高剂量组在15 min时T波位移值亦明显降低(P<0.05)。与正常组比较,其余各组血清ox-LDL、hs-CRP、ET-1含量均明显升高(P<0.05或P<0.01)。与模型组比较,中药高剂量和阿托伐他汀组ox-LDL含量明显降低,各给药组hs-CRP含量均明显降低,合心爽组和中药高剂量组ET-1含量明显降低(P<0.05)。结论清热涤痰解痉方能有效缓解垂体后叶素诱发高脂血症小鼠冠状动脉痉挛,其作用机制可能与降低血清ox-LDL、hs-CRP、ET-1水平有关。
Objective To observe the effect of Qingre Di Phlegm Prescription on coronary spasm induced by pituitrin in mice with hyperlipidemia and to explore its possible mechanism. Methods 140 C57BL / 6J mice were randomly divided into normal group, model group, combination group, atorvastatin group and traditional Chinese medicine high, medium and low dose group, 20 rats in each group. Except normal group, the other groups were given high-fat diet for 4 weeks. The Chinese herbal medicine high, medium and low dose groups were given heat-clearing and resolving phlegm anti-spasmodic granules 7.2,3.6,1.8 g / (kg · d), Heshun group were given Huxin cool 0.04 g / (kg · d) The atorvastatin calcium group was 0.013 g / (kg · d) in the Avastin group. The normal and model groups were treated with 0.5% sodium carboxymethyl cellulose solution once a day for 4 weeks. One hour after the last administration, the model of coronary artery spasm was established by intraperitoneal injection of pituitrin injection 60 U / kg. The electrocardiogram of Ⅱ lead was recorded at 10 min before injection and at 5, 10, 15 min after injection to calculate the T-wave displacement. After the test, serum levels of ox-LDL and hs -CRP), endothelin 1 (ET-1) levels. Results At 5, 10 and 15 min after the intraparenchymal injection of pituitrin, the T wave displacement of the electrocardiogram in the model group was significantly higher than that in the normal group (P <0.05). Compared with the model group, Compared with atorvastatin group, the T and D values in both low-dose, middle-dose and low-dose statin groups decreased significantly at 5, 10 and 15 min (P <0.05) The T wave displacement decreased significantly at 10 min, and T wave displacement at 15 min in the high-dose Chinese herbal medicine group also decreased significantly (P <0.05). Compared with the normal group, the contents of ox-LDL, hs-CRP and ET-1 in the other groups were significantly increased (P <0.05 or P <0.01). Compared with model group, the content of ox-LDL in high-dose Chinese medicine group and atorvastatin group decreased obviously, while the content of hs-CRP in each group decreased obviously, the content of ET-1 in Huxinchuan group and high-dose group decreased obviously (P <0.05). Conclusions: The method of clearing away heat, dissipating phlegm and resolving spasms can effectively relieve coronary vasospasm induced by vasopressin in hyperlipidemic mice. The mechanism may be related to the decrease of serum ox-LDL, hs-CRP and ET-1 levels.