论文部分内容阅读
目的 :探索国内不同种系动物诱发实验性变态反应性脑脊髓炎 ( EAE)的可能性 ,寻找诱导 EAE的合适实验动物 ,以推进国内临床神经免疫学的研究。方法 :用含异种动物脊髓的完全弗氏佐剂乳化物作为抗原 ,进行动物背部皮下多点注射 ,观察和比较动物的神经症状和病理变化。结果 :( 1)抗原诱导 EAE,如果发病 ,一般都在注射抗原 2周后开始出现症状 ,在注射后 19天左右症状最明显 ,且脑组织出现明显的炎性病理变化。 ( 2 ) SD大鼠的发病率为 13 %,Wistar大鼠的发病率为 3 0 %,昆明种 ( KM)小鼠的发病率为 0。 ( 3 )未发病的 Wistar大鼠再次注射抗原 ,观察 2个月 ,仍未发病。结论 :利用 Wistar大鼠制备 EAE动物模型在国内较适宜 ,而EAE发病的个体差异可能主要受遗传基因的控制。
OBJECTIVE: To explore the possibility of inducing experimental allergic encephalomyelitis (EAE) in different kinds of domestic animals and to find suitable experimental animals for inducing EAE so as to promote the clinical neuroimmunological research in China. Methods: Complete Freund’s adjuvant emulsion containing different kinds of spinal cord of animals was used as antigen to make multiple subcutaneous injections on the back of animals. The neurological symptoms and pathological changes of the animals were observed and compared. Results: (1) EAE induction by antigen was usually started 2 weeks after antigen injection. Symptoms were most obvious about 19 days after injection, and obvious inflammatory pathological changes occurred in brain tissue. (2) The incidence of SD rats was 13%, that of Wistar rats was 30%, and that of Kunming mice was 0. (3) The unwounded Wistar rats were re-injected with antigens, observed for 2 months and still not onset. Conclusion: The animal model of EAE using Wistar rats is more suitable in China, while the individual differences in the pathogenesis of EAE may be mainly controlled by the genetic.