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目前已有报道,单克隆抗体(McAb)能够特异地抑制某些病毒贴附特定细胞。作者发现筛选出来的McAb可通过阻断受体来抑制柯萨奇病毒某些原型和变异株感染HeLa细胞和人横纹肌肉瘤(RID)细胞,故可通过McAb深入研究对不同小RNA病毒的细胞受体本质。作者采用经HeLa细胞免疫的BALB/c小鼠脾细胞和骨髓瘤细胞融合产生McAb,并用改进的Campbell和Cords细胞保护试验检测。用CB3和CB3-RD病毒分别选出对应于HeLa和RD细胞受体的5种McAb。它们具有三种病毒细胞特征:(1)保护HeLa细胞不受CB3病毒感染;(2)保护RD细胞不受CB3-
It has been reported that the monoclonal antibody (McAb) can specifically inhibit certain viruses attached to specific cells. The authors found that McAb screened can block the receptor to inhibit certain prototype and variant strains of Coxsackie virus from infecting HeLa cells and human rhabdomyosarcoma (RID) cells, so that the cell receptors for different picornaviruses can be further studied by McAb Body essence. The authors produced McAbs by fusion of BALB / c mouse splenocytes and myeloma cells that were immunized with HeLa cells and tested with a modified Campbell and Cords cytoprotection assay. Five McAbs corresponding to HeLa and RD cell receptors were selected with CB3 and CB3-RD viruses, respectively. They have three viral cell features: (1) protection of HeLa cells from CB3 virus infection; (2) protection of RD cells from CB3-