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小脑顶核在运动控制和躯体平衡中起着重要作用.先前的研究揭示了小脑顶核接受下丘脑-小脑组胺能神经纤维的投射,但对支配小脑顶核的组胺能神经投射的功能作用目前还没有报道.本研究采用脑片制备观察了组胺对小脑顶核神经元放电活动的影响.47张小脑脑片记录了65个小脑顶核细胞,其中绝大部分细胞对组胺刺激表现出兴奋反应(58/65,89.2%).用低钙/高镁脑脊液灌流脑片,不能阻断这些神经元对组胺的兴奋反应(n=10),说明组胺对顶核神经元的兴奋作用是直接的突触后效应.组胺H2受体阻断剂ranitidine可阻断组胺对顶核细胞的兴奋效应(n=15),而H1受体阻断剂triprolidine(n=15)和chlorpheniramine(n=10)不能阻断组胺的兴奋效应.H2受体激动剂dimaprit可以模拟组胺对顶核细胞的兴奋效应(n=20),而H1受体激动剂2-pyridylethlamine不能引起小脑顶核神经元任何反应(n=16);并且,dimaprit对顶核的兴奋效应能被H2受体阻断剂ranitidine阻断(n=13),而不能被H1受体阻断剂triprolidine阻断(n=15).以上结果说明,组胺是经H2受体的介导兴奋小脑顶核神经元,提示下丘脑-小脑组胺能神经纤维可能通过其对小脑顶核神经元的兴奋性输入,调制经小脑顶核介导的感觉-运动整合过程.
The cerebellar fastigial nucleus plays an important role in motor control and somatic balance.Previous studies revealed that the parietal fastigial nucleus receives projection of hypothalamus-cerebellar histaminergic nerve fibers, but functions on histaminergic neurons that dominate the cerebellar fastigial nucleus Role has not been reported.In this study, the preparation of brain slices was used to observe the effect of histamine on the discharge activity of the cerebellar fastigial neurons.47 cerebellar cerebellar slices recorded 65 cerebellar fastigial nuclei, of which most of the cells were stimulated by histamine (58/65, 89.2%) showed that the hippocampal neurons were not blocked by perfusion of brain slices with low calcium / high magnesium cerebrospinal fluid (n = 10) (N = 15), whereas the H1 receptor blocker triprolidine (n = 15), the histamine H2 receptor blocker ranitidine, blocked the excitatory effect of histamine on the apomix cells ) And chlorpheniramine (n = 10) failed to block the excitatory effect of histamine.The H2 receptor agonist dimaprit mimicked the excitatory effect of histamine on the apomixocytes (n = 20), whereas the H1 receptor agonist 2-pyridylethlamine did not Induced any response in the fastigial nucleus neurons (n = 16); and, dimaprit did not Fen effect can be blocked by the H2 receptor blocker ranitidine (n = 13), but not by the H1 receptor blocker triprolidine (n = 15) .The above results suggest that histamine is mediated by the H2 receptor Guiding excited cerebellar fastigial nucleus neurons, suggesting that the hypothalamus - cerebellar histaminergic nerve fibers may be through its excitatory input to the cerebellar fastigial nucleus neurons, modulated by the cerebellar fastigial nucleus-mediated sensory-motor integration process.