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[目的]探讨血浆中苯并(a)芘二氢二醇环氧化物(BPDE)-白蛋白加合物作为焦炉工人多环芳烃长期暴露的生物标志物的可行性及其与尿中1-羟基芘(1-OHP)浓度之间的关系。[方法]采用反相高效液相色谱方法,对焦化厂37名焦炉作业工人(接触组)和47名非焦炉作业人员(对照组)血浆中BPDE-白蛋白加合物和尿中1-羟基芘浓度分别进行测定。[结果]接触组血浆中BPDE-白蛋白加合物和尿中1-羟基芘浓度的中位数分别为42．10fmol/mg白蛋白和5．46μmol/mol肌酐,均显著高于对照组(14．16μmol/mg白蛋白,2．96μmol/mol肌酐,P<0．01)。校正了个体的年龄、吸烟和饮酒状况后,接触组BPDE-白蛋白加合物浓度增高的危险度是对照组的1．79倍(P<0．05)。接触组1-羟基芘浓度增高的危险度是对照组的2．45倍(P<0．05)。并且在所有研究对象中,BPDE-白蛋白加合物与尿中1-羟基芘浓度存在显著的正相关关系(rs=0．349,P<0．01)。[结论]血浆中BPDE-白蛋白加合物与尿中1-羟基芘水平显著相关,BPDE-白蛋白加合物可作为反映多环芳烃较长时期暴露的接触生物标志物。
[Objective] To investigate the feasibility of benzo (a) pyrene dihydrogenated diol epoxide (BPDE) -albumin adduct in plasma as long-term biomarker of polycyclic aromatic hydrocarbons in coke oven workers and its relationship with urinary 1 - hydroxypyrene (1-OHP) the relationship between the concentration. [Method] The RPDEs of 37 coke oven workers (contact group) and 47 non-coke oven workers (control group) in the coke oven were used to measure the plasma levels of BPDE-albumin adduct and urine 1 - hydroxypyrene concentration were measured. [Results] The median concentrations of BPDE-albumin adduct and urinary 1-hydroxy-pyrene in the exposed group were 42.10 fmol / mg albumin and 5.46 μmol / mol creatinine, respectively, which were significantly higher than those in the control group 14.16 μmol / mg albumin, 2.96 μmol / mol creatinine, P <0.01). After adjusting for individual age, smoking and alcohol consumption, the risk of increased BPDE-albumin adduct concentrations in the exposure group was 1.79 times greater than that in the control group (P <0.05). Exposure to 1-hydroxypyrene was associated with a 2.45-fold increased risk (P <0.05) in the control group. And there was a significant positive correlation between BPDE-albumin adduct and urine 1-hydroxypyrene concentrations in all subjects (rs = 0.349, P <0.01). [Conclusion] BPDE-albumin adduct in plasma is significantly correlated with urinary 1-hydroxypyrene level. BPDE-albumin adduct can be used as a biomarker of exposure to PAHs exposed in a long period of time.