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目的建立恩他卡朋片含量测定的方法。方法色谱柱:Intersil C18(4.6 mm×250 mm,5μm),流动相:甲醇-乙腈-0.03 mmol.L-1磷酸二氢钾缓冲溶液(磷酸调节pH至2.75)(35∶25∶40,V/V/V),检测波长:310 nm,柱温:30℃,流速:1.0 mL.min-1。结果恩他卡朋的线性范围为10～50 mg.L-1(r=0.999 9),平均回收率为(99.74±0.69)%,RSD=0.68%(n=9)。结论本方法简便、准确、重现性好,可用于恩他卡朋片的含量测定。
Objective To establish a method for the determination of entrapin. METHODS Columns: Intersil C18 (4.6 mm × 250 mm, 5 μm), mobile phase: methanol-acetonitrile -0.03 mmol.L -1 potassium dihydrogen phosphate buffer solution / V / V), detection wavelength: 310 nm, column temperature: 30 ℃, flow rate: 1.0 mL.min-1. Results Entecavir had a linear range of 10-50 mg.L-1 (r = 0.999 9) with an average recovery of (99.74 ± 0.69)% and RSD = 0.68% (n = 9). Conclusion This method is simple, accurate, reproducible, and can be used for the determination of entacapone tablets.