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目的:建立符合中医辨证的血热出血大鼠模型,并对此模型进行初步的评价和验证。方法:采用腹腔注射内毒素和皮下注射干酵母相结合的方法建立血热出血大鼠模型,以体温差、凝血指标[凝血酶时间(TT)、凝血酶原时间(PT)、活化部分凝血酶时间(APTT)、纤维蛋白原(FIB)]、病理组织学观察为指标进行初步评价,并以清热凉血药生地黄对此模型进行验证。结果:与正常组相比,模型组体温显著升高(P<0.05),而生地黄高剂量组的体温则显著下降(P<0.05);模型组PT延长(P<0.05),FIB升高(P<0.05),TT缩短(P<0.05),生地黄低剂量组可使TT显著延长(P<0.05),使FIB显著降低(P<0.05);模型组大鼠主要脏器发生明显病理学变化:瘀血、凝血、微血栓,出血等,而生地黄对这些病理变化有明显的改善作用。结论:本研究所采用的复合因素造模法可成功建立大鼠血热出血模型,可为血热出血证、清热凉血药的实验研究奠定基础。
OBJECTIVE: To establish a rat model of hemorrhagic fever with blood stasis that accords with syndrome differentiation of traditional Chinese medicine and make a preliminary evaluation and verification of this model. Methods: A rat model of hemorrhagic haemorrhagic fever was established by intraperitoneal injection of endotoxin and subcutaneous injection of dry yeast. The body temperature and blood coagulation index [thrombin time (TT), prothrombin time (PT), activated partial thrombin Time (APTT), fibrinogen (FIB)], histopathological observations as an indicator of initial evaluation, and the heat-clearing and cooling blood drug Rehmannia model validation. Results: Compared with the normal group, the body temperature of the model group was significantly increased (P <0.05), while the body temperature of the high dose group was significantly decreased (P <0.05). The model group PT was prolonged (P <0.05) (P <0.05), TT shortened (P <0.05), and the low dose of Dihuang Rehmanniae could prolong the TT significantly (P <0.05) and make the FIB significantly lower (P <0.05) Neo-Confucianism changes: blood stasis, blood clotting, micro thrombosis, bleeding, etc., while Rehmannia has a significant improvement on these pathological changes. Conclusion: The composite factor modeling method used in this study can successfully establish a rat model of hemorrhagic fever and blood hemorrhage, which may provide the basis for the experimental study of hemorrhagic fever with blood-heat and clearing heat and cooling blood.