论文部分内容阅读
玉米大斑病是严重危害玉米生产的一个世界性真菌病害。由于玉米大斑病菌 (Exserohilumturcicum)在无性生长过程中迅速产生黑色素 ,致使原生质体难以分离。测试了包括Fungase、Funcelase、Novozyme、Glucanex、Driselase、Uskizyme、Kitalase在内的 7种细胞壁降解酶及其组合、病原菌菌株和培养基对原生质体分离效果的影响。结果表明菌株的培养形态和菌丝的生长状态显著影响原生质体的分离效率 ;酶组合Kitalase +Glucanex +Driselase ,Kitalase+Glucanex和Kitalase +Uskizyme能够有效地分离玉米大斑病菌的原生质体。初步的转化试验表明 ,质粒pAN71可以用于该病原菌的转化。这些结果将为E .turcicum和Exserohium属其它真菌的基因克隆提供一些有用的信息
The maize leaf spot is a worldwide fungal disease that seriously damages the production of corn. Exserohilumturcicum rapidly produces melanin during asexual growth, making protoplasts difficult to isolate. Seven cell wall degrading enzymes, including Fungase, Funcelase, Novozyme, Glucanex, Driselase, Uskizyme, Kitalase, and combinations thereof, pathogenic bacteria strains and media were tested for their effect on protoplast isolation. The results showed that the morphology of mycelia and the growth of mycelia significantly affected the isolation efficiency of protoplasts. The enzyme combinations Kitalase + Glucanex + Driselase, Kitalase + Glucanex and Kitalase + Uskizyme were effective in isolating the protoplasts of S. turcica. Preliminary transformation experiments show that the plasmid pAN71 can be used for the transformation of the pathogen. These results will provide some useful information for the gene cloning of other fungi of the genus E. Turcicum and of the genus Exserohium