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[目的]观察甘氨鹅脱氧胆酸钠(glycochenodeoxycholate,GCDCA)诱导人肝癌细胞SMMC7721凋亡过程中caspase3,9活性变化,探讨GCDCA诱导肝癌细胞凋亡的机制。[方法]体外培养SMMC7721细胞,GCDCA为处理因素,Annexin V—FITC/PI双染色法结合流式细胞技术仪检测细胞凋亡率,比色法测定caspase3,9活性。[结果]200μmol/L的GCDCA处理SMMC7721细胞24h,48h,72h后,其细胞凋亡率明显增加;caspase3,9活性表达水平明显升高,并与GCDCA呈浓度依赖性,与对照组相比差异有统计学意义(P﹤0.05)。[结论]GCDCA可明显诱导肝癌SMMC7721细胞凋亡,caspase3,9参与GCDCA诱导SMMC7721细胞凋亡调控。
[Objective] To observe the changes of caspase 3 and 9 activities induced by glycochenodeoxycholate (GCDCA) in human hepatoma SMMC7721 cells and to explore the mechanism of GCDCA inducing apoptosis of hepatocellular carcinoma cells. [Method] SMMC7721 cells were cultured in vitro, GCDCA was the treatment factor, apoptosis rate was detected by Annexin V-FITC / PI double staining combined with flow cytometry, and the activity of caspase 3 and 9 was determined by colorimetry. [Result] The apoptosis rate of SMMC7721 cells treated with 200μmol / L GCDCA for 24h, 48h and 72h was significantly increased, and the expression of caspase 3 and 9 significantly increased, which was in a concentration-dependent manner with GCDCA compared with the control group There was statistical significance (P <0.05). [Conclusion] GCDCA can obviously induce the apoptosis of SMMC7721 cells, and caspase3,9 is involved in the regulation of apoptosis induced by GCDCA in SMMC7721 cells.