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GBV-C/HGV RNA transcripts were transcribed in vitro from a single GBV-C/HGV full-length cDNA clone pHGVqz, and injected directly into the liver of Macaca mulatta to test their infectivity. Serum samples from the experimental Macaca mulatta were collected weekly after injection to detect ALT, anti-GBV-C/HGV and GBV-C/HGV RNA. At week 6 post injection, the liver tissues of the infected Macaca mulatta were dissected through operation for histological examination. The results showed that ALT level of the Macaca mulatta remained normal and anti-GBV-C/HGV kept negative after infection. GBV-C/HGV RNA was detected positve from week 1 through week 14 post injection. The liver biopsy showed light viral-hepatitis like histological changes. From the preliminary results, we concluded that the in vitro transcripts of GBV-C/HGV may be infectious. Further studies are under way to confirm the conclusion.
GBV-C / HGV RNA transcripts were transcribed in vitro from a single GBV-C / HGV full-length cDNA clone pHGVqz, and injected directly into the liver of Macaca mulatta to test their infectivity. Serum samples from the experimental Macaca mulatta were collected weekly after injection to detect ALT, anti-GBV-C / HGV and GBV-C / HGV RNA. At week 6 post injection, the liver tissues of the infected Macaca mulatta were dissected through operation for histological examination. The results showed that ALT level of the Macaca mulatta remained normal and anti-GBV-C / HGV kept negative after infection. GBV-C / HGV RNA was detected positve from week 1 through week 14 post injection. The liver biopsy showed light viral-hepatitis like histological changes. Further studies are under way to confirm the conclusion.