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以滇西北地区11个羊肚菌居群56个羊肚菌样品为研究材料,应用ISSR分子标记方法,进行了遗传多样性与亲缘关系分析。结果表明,11个多态性ISSR引物对全部试验样品进行PCR扩增,共获得88条稳定的条带,其中多态性条带70条(占79.55%)。应用软件POPGENE 32分析得出11个羊肚菌居群间遗传距离的变化范围在0.1715～0.4853之间,相似系数的变化范围在0.6155～0.8424之间。遗传分化分析表明,61.35%的变异存在于居群间,38.65%的变异存在于居群内。对56个羊肚菌样品进行分子系统聚类分析(UPGMA)将资源分为三大组,对11个羊肚菌居群进行亲缘关系聚类分析将居群分为两大类群,聚类结果与地理距离有明显的相关性。
In this study, 56 Morchella esculenta samples collected from 11 Morchella esculenta communities in northwestern Yunnan were used to analyze the genetic diversity and genetic relationship using ISSR molecular markers. The results showed that 11 polymorphic ISSR primers were used for PCR amplification of all the test samples. A total of 88 stable bands were obtained, of which 70 were polymorphic (79.55%). The analysis of POPGENE 32 software showed that the genetic distances among 11 Morchellaes ranged from 0.1715 to 0.4853, and the similarities ranged from 0.6155 to 0.8424. Genetic differentiation analysis showed that 61.35% of the variation existed among the populations and 38.65% of the variation existed within the population. Fifty-six Morchella samples were divided into three groups according to molecular phylogenetic tree analysis (UPGMA). Cluster analysis of 11 Morchella species divided the populations into two groups. The clustering results There is a clear correlation with geographical distance.