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利用肥胖患者血液和肥胖动物模型研究Fas凋亡抑制分子FAIM 1与单纯性肥胖的关系,为揭示单纯性肥胖发生的分子机理和诊治肥胖提供新的实验基础。检测40例单纯性肥胖患者和17例正常者血液白细胞的FAIM 1蛋白的表达量,分析FAIM 1与单纯性肥胖的相关性。为进一步揭示FAIM 1与单纯性肥胖发生、发展的内在关系,利用高脂饲料建立肥胖模型,并测定肥胖组和对照组大鼠的体重和血糖水平;造模成功的肥胖组大鼠和对照组大鼠禁食12h,麻醉心脏取血,分析血脂中总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白-胆固醇(HDL-C)和低密度脂蛋白-胆固醇(LDL-C)的变化;取肥胖组和对照组大鼠的附睾脂肪垫和肾周脂肪,分析重量变化;取肥胖组和对照组大鼠,利用Western-blot检测FAIM 1及胰岛素受体β(IRβ)的表达变化。实验结果表明:与正常者相比,单纯性肥胖患者血液白细胞的FAIM 1表达量平均减少36.4%;与对照组相比,肥胖组大鼠血清TC、TG和LDL-C分别增加37.1%、25.6%和39.1%,而HDL-C则降低33.3%;肥胖组大鼠附睾脂肪垫和肾周脂肪分别是对照组的1.85倍和2.24倍;与对照组相比,肥胖组大鼠肝脏FAIM 1、IRβ的表达量分别降低45.9%和32.6%,与临床血液样本的检测结果一致。研究表明,FAIM 1表达与单纯性肥胖发生具有显著的负相关性,FAIM 1可成为单纯性肥胖诊断和治疗的新靶点。
To study the relationship between FAIM 1 and simple obesity in obese patients with blood and obesity animal model, and to provide a new experimental basis for revealing the molecular mechanism of obesity and diagnosis and treatment of obesity. The expression of FAIM 1 protein in 40 patients with simple obesity and 17 normal subjects was detected, and the correlation between FAIM 1 and simple obesity was analyzed. In order to further reveal the relationship between the occurrence and development of FAIM 1 and simple obesity, the model of obesity was established by using high-fat diet and the body weight and blood glucose level of obese and control rats were determined. The obese rats and control group The rats were fasted for 12 hours, and blood was collected from the anesthetized hearts. The contents of total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDL-C) and low density lipoprotein cholesterol (LDL- The fat epididymal fat pad and perirenal adipose tissue of the rats in the obesity group and the control group were analyzed for weight change. The rats in the obesity group and the control group were subjected to Western-blot to detect the expression of FAIM 1 and insulin receptor β (IRβ) Variety. The results showed that compared with normal subjects, the expression of FAIM 1 in leukocytes of patients with simple obesity was reduced by 36.4% on average compared with that of control subjects. Compared with control subjects, the levels of TC, TG and LDL-C in obese rats increased by 37.1%, 25.6 %, And 39.1%, while the HDL-C decreased by 33.3%; fat epididymal fat pad and perirenal fat were 1.85 times and 2.24 times of the control group respectively; compared with the control group, the obese rats liver FAIM 1, The expression of IRβ decreased by 45.9% and 32.6% respectively, which was consistent with the results of clinical blood samples. Studies have shown that, FAIM 1 expression and simple obesity has a significant negative correlation, FAIM 1 can be a new target for the diagnosis and treatment of simple obesity.