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目的调查昆明市2011年HIV耐药株的传播情况。方法采用WHO推荐的HIVDR-TS法进行调查,连续收集昆明市2011年16~25岁间新确诊的82份HIV感染者血浆样本进行HIV-1基因型检测及耐药株传播水平分析。结果54份样本成功扩增并测序。其中满足耐药传播水平分析的前41条序列中,18份样本(43.9%)为CRF07_BC亚型,16份为CRF08_BC(39.0%),另外7份为CRF01_AE(17.1%)。41份样本中,只有1条序列的核苷类逆转录基因有耐药突变(T69D),耐药传播水平的分析表明昆明HIV耐药株低流行率低于5%。结论耐药警戒线监测结果说明昆明市HIV耐药株为低流行水平,应继续定期进行HIV-1耐药警戒线监测。
Objective To investigate the spread of HIV-resistant strains in Kunming in 2011. Methods HIVDR-TS method recommended by the WHO was used to investigate the HIV-1 genotypes and the transmission of drug-resistant strains in 82 newly diagnosed HIV-infected patients from 16 to 25 years of age in Kunming in 2011. Results 54 samples were successfully amplified and sequenced. Of the first 41 sequences that met the analysis of drug resistance, 18 samples (43.9%) were CRF07_BC subtypes, 16 were CRF08_BC (39.0%) and the other 7 were CRF01_AE (17.1%). Among 41 samples, only one nucleotide reverse transcriptase gene had a resistance mutation (T69D). The analysis of drug resistance transmission showed that the prevalence of HIV-resistant strains in Kunming was lower than 5%. Conclusion The monitoring results of drug-resistant cordon show that HIV-resistant strains of Kunming are low-prevalence level, and HIV-1 drug resistance monitoring should be continued on a regular basis.