A biostratigraphic study of calcareous nannofossils of sediments from Lingfeng No. 1 well in the East China Sea was carried out to provide more detailed nannofossil data and a stratigraphic correlation among offshore wells located in the East China Sea. 14 Paleocene to Pleistocene calcareous nannofossil zones were recognized based on a detailed examination. According to these nannofossil data, the Paleocene/Eocene and Pliocene/Pleistocene boundaries, as well as the Miocene base were determined. Oligocene NP23 and Miocene NN1 zones are firstly recognized by this study.The existence of Miocene NN1 at this well suggests that the Miocene transgression began in the Early Miocene in this area. The records of NP23 indicate that the marine Oligocene sediments exist in the series of Lingfeng wells, but probably as very thin layers.