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从大豆胚轴提取总糖苷后,采用C18反相柱层析法分离大豆异黄酮糖苷,再将其水解制备成异黄酮苷元,并对大豆异黄酮苷元的抗脂蛋白氧化作用进行了实验研究。12名健康受试者空腹食用大豆异黄酮苷元前后抽取静脉血制备血浆脂蛋白,观察血浆脂蛋白过氧化程度及氧化易感性的变化。结果表明,大豆异黄酮苷元食用后受试者血浆VLDL、LDL和HDL过氧化脂质水平明显降低;在体外进行氧化修饰时,LDL氧化延滞时间明显延长,LDL氧化易感性下降。大豆异黄酮苷元可增强血浆抗氧化能力,尤其增强LDL抗氧化修饰能力,因此能够起到防治心血管疾病的积极作用。
After total glycoside was extracted from the soybean hypocotyl, the soy isoflavone glucoside was separated by C18 reverse phase column chromatography, then hydrolyzed to prepare isoflavone aglycone, and the oxidation of soybean isoflavone aglycone was tested the study. Twelve healthy subjects were fed with fasting soy isoflavone agar extract before and after venous blood to prepare plasma lipoprotein, and observed the changes of plasma lipoprotein peroxidation and oxidative susceptibility. The results showed that the levels of lipid peroxidation lipid in VLDL, LDL and HDL were significantly decreased in subjects receiving soy isoflavone aglycone. Oxidative modification prolonged LDL oxidation delay and decreased LDL oxidation susceptibility in vitro. Soy isoflavone aglycone can enhance plasma antioxidant capacity, in particular, enhance the ability of LDL anti-oxidative modification, it can play a positive role in the prevention and treatment of cardiovascular disease.