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以红王子锦带带腋芽的茎段为外植体进行离体培养基的筛选。结果表明 :红王子锦带的离体培养以MS、WPM为基本培养基 ,附加适当比例的细胞分裂素(0.1～1mg·L-16 -BA)和生长素(0.01～0.1mg·L-1NAA)为宜。本试验采用方差分析及多重比较方法对试验数据进行处理 ,筛选出红王子锦带各培养阶段最佳培养基分别为:(1)外植体诱导培养基(A2) :MS +6 -0.1mg·L -1BA +0.01mg·L -1NAA ;(2)分化及继代培养基(B3) :MS +0.5mg·L -16 -BA +0.05mg·L -1NAA ;(3)生根培养基(C2) :WPM +0.4mg·L -1NAA。
The stems of axillary buds of Prunus mandshurica were used as explants for the screening of in vitro culture medium. The results showed that in vitro culture of P. kaempferi with MS and WPM as the basic medium, the appropriate proportion of cytokinin (0.1-1 mg · L-16 -BA) and auxin (0.01-0.1 mg · L -1 NAA ) Is appropriate. In this study, the variance analysis and multiple comparison methods were used to deal with the experimental data. The optimal media of each stage of culture were selected as follows: (1) explants induction medium (A2): MS +6 -0.1mg · L -1 BA + 0.01 mg · L -1 NAA; (2) Differentiation and subculture medium (B3): MS + 0.5 mg · L -16 -BA + 0.05 mg · L -1 NAA; C2): WPM + 0.4 mg · L -1 NAA.