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目的 :研究人红细胞 (humanerythrocytes,HRC)和兔小肠刷状缘囊泡 (rabbitintestinalbrush bordermem branevesicles,BBMV)对稀土离子Ln3 + 的摄入 ,以及牛血清白蛋白 (bovineserumalbumin ,BSA)、柠檬酸根 (Cit3 -)和二价金属离子 (M2 + )的影响。方法 :在溶液中有或无BSA (或Cit3 -、Cu2 + 、Co2 + 、Zn2 + 、Cd2 + 、Pb2 + )的条件下 ,在HRC或提取的BBMV与Ln3 + 温育后 ,用ICP或ICP MS测定HRC胞浆及膜或BBMV中Ln3 + 及M2 + 的含量。结果 :被研究的Ce3 + 、Gd3 + 、Yb3 + 离子都能跨膜进入人红细胞 ,BSA对Ce3 + 和Yb3 + 的跨膜输运有促进作用 ,但对Gd3 + 无影响。Pb2 + 、Cu2 + 、Co2 + 、Zn2 + 对HRC膜结合Ce3 + 量无影响 ,但Cu2 + 促进Ce3 + 跨膜进入细胞内。Ce3 + 促进细胞对Co2 + 摄入 ,但Co2 + 和Zn2 + 几乎完全抑制了Ce3 + 的跨膜输运。BBMV可以结合Ce3 + 、Gd3 + 、Lu3 + 和Cu2 + 、Pb2 + 、Cd2 + ;Cit3 -抑制Ln3 + 的结合 ,抑制率随 [Ln(Cit) 2 ]3 -稳定性增加而增加 ;Ce3 + 和Pb2 + 或Cd2 + 之间相互抑制其与BBMV的结合 ,但Cu2 + 促进Gd3 + 与BBMV的结合。结论 :在HRC和BBMV对Ln3 + 和M2 + 的摄取中 ,Ln3 + 和M2 + 间存在着某些拮抗或协同作用 ,这些作用与Ln3 + 和M2 + 的性质有关
OBJECTIVE: To investigate the effects of human erythrocyte humanerythrocytes (HRC) and rabbit intestinalbrush bordermem branevesicles (BBMV) on the uptake of Ln3 + and bovine serum albumin (BSA), citrate (Cit3 - ) And divalent metal ions (M2 +). Methods: After incubation of HRC or extracted BBMV with Ln3 + in the presence or absence of BSA (or Cit3 -, Cu2 +, Co2 +, Zn2 +, Cd2 +, Pb2 +) in solution, MS assay of the content of Ln3 + and M2 + in the cytoplasm and membrane or BBMV of HRC. Results: Ce3 +, Gd3 + and Yb3 + ions all transmembrane into human erythrocytes. BSA could promote the transmembrane transport of Ce3 + and Yb3 +, but had no effect on Gd3 +. Pb2 +, Cu2 +, Co2 + and Zn2 + had no effect on Ce3 + content of HRC membrane, but Cu2 + promoted Ce3 + transmembrane entry into cells. Ce3 + promoted cell uptake of Co2 +, but Co2 + and Zn2 + almost completely inhibited the transmembrane transport of Ce3 +. BBMV could inhibit the binding of Ln3 + with Ce3 +, Gd3 +, Lu3 + and Cu2 +, Pb2 +, Cd2 + and Cit3. The inhibition rate increased with the increase of [Ln (Cit) 2] Pb2 + or Cd2 + mutually inhibited their binding to BBMV, but Cu2 + promoted the binding of Gd3 + to BBMV. CONCLUSIONS: There is some antagonism or synergism between Ln3 + and M2 + in uptake of Ln3 + and M2 + by HRC and BBMV and these effects are related to the properties of Ln3 + and M2 +