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40只健康雄性Wistar大鼠随机分为对照组(C组)和照射组(R组),R组行6MV-X线全胸野照射,用HE和Masson染色观察病理改变,免疫组化法分析大鼠急性放射性肺损伤过程中表皮生长因子受体(EGFR)在肺组织中的表达。发现照射后的1周始肺泡腔有炎性细胞渗出,继而间质水肿,4、8周出现肺泡腔变小甚至结构破坏,局部实变,肺间质出现胶原纤维;R组各时间段肺泡灌洗液的细胞总数较C组增高;C组比R组在第4、8周EG-FR表达明显增强。认为EGFR参与放射性肺损伤的发生、发展过程,为进一步探讨放射性肺损伤的发病机制提供了实验依据。
Forty healthy male Wistar rats were randomly divided into control group (group C) and irradiation group (group R). Group R was irradiated with 6MV-X line in full chest field. The pathological changes were observed by HE and Masson staining. Immunohistochemistry Expression of Epidermal Growth Factor Receptor (EGFR) in Lung Tissue During Acute Lung Injury in Rats. Found 1 week after irradiation inflammatory cell exudation of the alveolar space, and then interstitial edema, 4,8 weeks alveolar cavity diminished or even structural damage, local consolidation, interstitial collagen fibers appear; R group at each time period The total number of cells in alveolar lavage fluid was higher than that in group C. The expression of EG-FR in group C was significantly higher than that in group R at 4 and 8 weeks. That EGFR involved in the occurrence and development of radiation-induced lung injury, to provide experimental evidence for further explore the pathogenesis of radiation-induced lung injury.