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目的 探讨kruppel样因子5(KLF5)和整合素亚单位α3(ITGA3)在喉癌中的表达及与患者预后的关系.方法 选取2012年2月-2013年6月本院收治的76例喉癌患者作为研究对象,收集癌组织标本76例为研究组,对应癌旁正常组织标本76例为对照组,采用实时荧光定量PCR技术(qRT-PCR)检测所有标本中KLF5、ITGA3表达水平,并探讨二者与临床病理参数及预后的关系.结果 研究组患者KLF5表达水平低于对照组,ITGA3表达水平高于对照组,差异均有统计学意义(P＜0.05).喉癌组织中KLF5阳性表达率为26.67％,明显低于癌旁组织中的66.67％,差异有统计学意义(P＜0.05);喉癌组织中ITGA3阳性表达率为69.33％,明显高于癌旁组织中的18.67％,差异有统计学意义(P＜0.05).KLF5表达与性别、年龄、临床分型、组织学分化无关(P＞0.05),与TNM分期、淋巴结转移、复发有关(P＜0.05);ITGA3表达与性别、年龄、临床分型无关(P＞0.05),与组织学分化、TNM分期、淋巴结转移、复发有关(P＜0.05).Pearson相关性分析显示,喉癌组织中KLF5和ITGA3表达呈负相关性(r=-0.851,P=0.000).KLF5低表达组患者中位生存时间为47.48个月,明显低于高表达组患者54.56个月,差异有统计学意义(P＜0.05);ITGA3低表达组患者中位生存时间为53.45个月,明显高于高表达组患者的45.67个月,差异有统计学意义(P＜0.05).结论 喉癌组织中KLF5呈低表达,ITGA3高表达,且与患者TNM分期、淋巴结转移、复发有关,同时与患者预后也密切相关.“,”Objective To investigate the expressions of Kruppel-like factor 5 (KLF5) and integrin subunit α3 (ITGA3) in laryngeal cancer and their clinical significances. Methods 76 patients with laryngeal cancer admitted to the Oncology Department of our hospital from February 2012 to June 2013 were selected as the study subjects. 76 cases of cancer tissue specimens were collected as the study group, and 76 cases of normal tissue specimens adjacent to cancer were collected as the control group. The expression levels of KLF5 and ITGA3 in all samples were detected by real-time fluorescence quantitative PCR (qRT-PCR); the relationships between the two with clinicopathological parameters and prognosis were also discussed. Results The expression of KLF5 in the study group was lower than that in the control group (P＜0.05); the expression level of ITGA3 was higher than that in the control group (P＜0.05); the positive expression rate of KLF5 in laryngeal carcinoma was 26.67％, which was significantly lower than that in adjacent tissues 66.67％ (P＜0.05); the positive expression rate of ITGA3 in laryngeal carcinoma was 69.33％, which was significantly higher than 18.67％ in adjacent tissues (P＜0.05). The expression of KLF5 was not related to sex, age, clinical classification, and histological differentiation (P＞0.05), but to TNM stage, lymph node metastasis, and recurrence (P＜0.05); the expression of ITGA3 was not related to gender, age, and clinical classification (P＞0.05), but related to histological differentiation, TNM stage, lymph node metastasis, and recurrence (P＜0.05). Pearson correlation analysis showed that the expressions of KLF5 and ITGA3 in laryngeal cancer were negatively correlated (r=0.851, P=0.000); the median survival time of KLF5 low expression group was 47.48 months, which was significantly lower than 54.56 months of high expression group (P＜0.05), the median survival time of patients with low expression of ITGA3 was 53.45 months, which was significantly higher than 45.67 months of patients with high expression of ITGA3 (P＜0.05). Conclusion KLF5 was low expressed and ITGA3 was high expressed in laryngeal cancer, and was related to ITNM stage, lymph node metastasis, and recurrence; at the same time, it was closely related to the prognosis of patients.