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目的建立奥氮平诱导性肥胖大鼠模型并观察奥氮平诱导所致肥胖大鼠脂肪组织中肿瘤坏死因子-α(TNF-α)水平变化。方法 40只大鼠随机分为两组,研究组在普通饲料喂养基础上灌胃奥氮平(1.2 mg/kg·d),持续喂养4周建立奥氮平诱导肥胖大鼠模型(20只),对照组普通饲料持续喂养4周(20只)。采用酶联免疫测定法测定各组大鼠血清中胰岛素(INS)和TNF-α的含量,放射免疫法检测脂肪组织中TNF-α的含量,生化比色法测定血清葡萄糖(FBS)含量。结果灌胃2、3、4周研究组大鼠体质量、血清细胞因子TNF-α较灌胃1周增加(P<0.05);灌胃4周研究组大鼠脂肪组织TNF-α较灌胃1周增加(P<0.05)。灌胃4周研究组大鼠体质量、血糠、血清胰岛素、血清甘油三酯、总胆固醇、低密度脂蛋白、血清及脂肪组织TNF-α含量高于对照组(P<0.05),高密度脂蛋白水平低于对照组(P<0.05)。结论奥氮平可以导致大鼠肥胖,血脂、胰岛素和糖代谢紊乱,升高脂肪组织和血清TNF-α水平。
Objective To establish an olanzapine-induced rat model of obesity and observe the changes of tumor necrosis factor-α (TNF-α) in obese rats induced by olanzapine. Methods Forty rats were randomly divided into two groups. The study group was orally administered olanzapine (1.2 mg / kg · d) , The control group of normal feed for 4 weeks (20). Serum insulin (INS) and TNF-α levels were measured by enzyme-linked immunosorbent assay (ELISA), radioimmunoassay was used to detect the content of TNF-α in adipose tissue and serum creatinine (FBS) was determined by biochemical colorimetry. Results After 2, 3, and 4 weeks of treatment, body weight and serum cytokine TNF-α level in the study group were significantly increased (P <0.05) 1 week increased (P <0.05). The body weight, blood bran, serum insulin, serum triglyceride, total cholesterol, low density lipoprotein, serum and adipose tissue TNF-αlevels in the study group were significantly higher than those in the control group (P <0.05) Lipoprotein levels were lower than the control group (P <0.05). Conclusion Olanzapine can cause obesity, dyslipidemia, insulin and glucose metabolism in rats and increase the level of TNF-α in adipose tissue and serum.