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用 EI(0.5%,V/V)和 X 射线(25kR)处理三个水稻品种的干种子,在M_2—M_4代筛选出雄性不育突变体。本试验将雌性器官没有畸型、自花授粉完全不育的植株,称为“雄性不育突变体”。这些植株的花药不开裂,含的花粉粒很少或没有完好的花粉粒。ET 处理,三个品种诱发雄性不育突变体的频率为0.30—0.38%,共8个突变体。而 X 射线辐照诱变未见效果。突变体与其原品种杂交,在自花授粉和自由授粉条件下,F_1代出现正常的可育种子。F_2代的分离比例表明,除一个突变体外,其他突变体都由一个隐性基因控制。这些隐性基因的等位测定表明,至少有6个不同位点控制不同的雄性不育。结果表明,ET 对诱发雄性不育突变体具有较高的效果,也说明水稻上有相当多的位点与雄性不育有关。
The dry seeds of three rice cultivars were treated with EI (0.5%, V / V) and X-ray (25 kR), and male sterile mutants were screened on M_2-M_4 generation. In this experiment, the female organ is not deformed, self-pollination completely sterile plants, known as “male sterile mutant.” The anthers of these plants are not cracked, contain little or no intact pollen grains. ET treatment, the frequency of male-sterile mutant induced by three cultivars was 0.30-0.38%, a total of 8 mutants. The X-ray irradiation induced no effect. The mutants crossed with their original breed, and under the conditions of self-pollination and free pollination, F_1 generation of normal fertile seeds. The F 2 generation segregation ratio showed that all the mutants were controlled by one recessive gene except for one mutant. Allelic measurements of these recessive genes indicate that at least 6 different loci control different male sterility. The results showed that ET had high effect on inducing male sterility mutants, and also showed that there were a considerable number of sites on rice related to male sterility.