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目的:构建5-羟色胺1A(5-HT_(1A))受体细胞膜色谱模型并对其药物筛选能力进行考察。方法:用体外培养的T淋巴细胞,以硅胶为载体制备5-HT_(1A)受体细胞膜固定相,构建5-HT_(1A)受体细胞膜色谱模型,以5-羟色胺(5-HT)、丁螺环酮和8-羟基-丙胺-四氢萘(8-OH-DPAT)3种5-HT_(1A)受体激动剂验证该色谱模型的有效性,并以分别含有上述3种激动剂的枸杞提取液考察该模型的药物筛选能力。结果:3种激动剂在硅胶柱与细胞膜色谱模型上的色谱行为存在显著差异,利用制备的细胞膜色谱模型可以快速准确地将每种激动剂从混合溶液中筛选出来。结论:5-HT_(1A)受体细胞膜色谱模型建立成功,且具备较强的药物筛选能力,可用于特异性地筛选具有5-HT_(1A)受体结合能力的药物。
OBJECTIVE: To construct a chromatographic model of 5-hydroxytryptamine 1A (5-HT_ (1A)) receptor cell membrane and investigate its drug screening ability. Methods: The 5-HT 1A receptor cell membrane phase was prepared from 5-HT 1A receptor membrane by using T lymphocytes cultured in vitro. Buspirone and 8-hydroxy-propylamine-tetrahydronaphthalene (8-OH-DPAT) three kinds of 5-HT 1A receptor agonists to verify the validity of the chromatographic model, and respectively containing the above three kinds of agonists Of the medlar extract to examine the model of drug screening ability. Results: The chromatographic behaviors of the three agonists in silica gel column and cell membrane chromatography were significantly different. The prepared cell membrane chromatography model was used to select each agonist rapidly and accurately from the mixed solution. CONCLUSION: The 5-HT 1A receptor cell membrane chromatographic model has been successfully established and has strong drug screening ability, which can be used to screen drugs with 5-HT 1A receptor binding specificity.