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作者参照Gottesfeld的DNaseⅡ温和消化、配合Mg~(++)溶解的方法,结合本实验室条件加以改进,将大鼠肝染色质分离成S_2、P_1、P_2三个组份。比较分析了它们的化学组成,模板活性及对DNase Ⅰ消化的敏感性。结果显示:S_2染色质组份富含RNA和非组蛋白,具有高模板活性和高度的DNaseⅠ消化敏感性,其RNA含量为P_1、P_2RNA含量的2倍以上,S_2的模板活性分别为P_1、P_2的7.7倍和3.2倍,它对DNase Ⅰ的消化敏感性分别是P_1、P_2组份的20倍和7倍以上。说明此法分离的S_2染色质组份为肝细胞核内的转录活性染色质,而P_1、P_2组份为转录非活性染色质。
According to Gottesfeld DNase Ⅱ mild digestion, combined with Mg ~ (++) dissolution method, combined with the laboratory conditions to be improved, the rat liver chromatin was separated into S_2, P_1, P_2 three components. Their chemical composition, template activity and sensitivity to DNase I digestion were compared. The results showed that the S 2 chromatin component was rich in RNA and non-histone proteins and had high template activity and high DNase Ⅰ digestion sensitivity. The RNA content of S 2 was more than 2 times higher than that of P 1 and P 2 RNA. The S 2 template activity was P 1, P 2 7.7 times and 3.2 times, respectively. Its digestive sensitivity to DNase Ⅰ was 20 times and 7 times higher than that of P_1 and P_2, respectively. S 2 chromatin components isolated from this method are transcriptional active chromatin in the nucleus of hepatocytes, while P 1 and P 2 components are transcriptional inactive chromatin.