从Th1/Th2细胞代表性细胞因子IFN γ/IL 4和IL 12水平的变化分析SLE患者Th1/Th2细胞的偏移现象。本实验通过半定量PCR法和ELISA酶联免疫检测法检测上述三种细胞因子含量。实验结果显示 ,SLE患者IL 4/IFN γ基因水平比值为0 32 8,高于对照组 (0 0 7)。ELISA检测显示IL 12在SLE患者血清中为 (38 39± 15 1)pg/ml,低于对照组 (84 97± 13 7)pg/ml(P <0 0 5 )。结论 :SLE患者Th1/Th2细胞因子平衡失调 ,IL 4细胞因子基因水平增高 ,同时血清中IL 12水平降低。 The shift of Th1 / Th2 cells in SLE patients was analyzed from the changes of the levels of Th1 / Th2 cytokine IFNγ / IL4 and IL12. In this experiment, the above three cytokines were detected by semi-quantitative PCR and ELISA enzyme-linked immunosorbent assay. The experimental results showed that the level of IL 4 / IFN γ gene in SLE patients was 0 32 8, which was higher than that of the control group (0 0 7). The level of IL-12 in serum of patients with SLE was (38 39 ± 15 1) pg / ml, which was lower than that of the control group (84 97 ± 13 7) pg / ml by ELISA (P 0 05). CONCLUSIONS: Th1 / Th2 cytokines are imbalanced in SLE patients, IL 4 cytokine gene level is elevated, and serum IL 12 level is decreased.