目的建立一种快速、高效的减肥药物体外筛选模型,并对该模型进行初步评价。方法用油酸诱导SD大鼠原代成熟脂肪细胞建立肥胖细胞模型,选用阳性药物异丙肾上腺素和已明确有减肥作用的功能因子染料木素、咖啡因及阴性对照物姜黄素对该肥胖模型进行评价。结果试验所选用的阳性受试物均能显著刺激脂肪细胞发生脂解,而阴性受试物无脂解作用。结论该模型可以用于减肥药物的体外快速筛选。 Objective To establish a rapid and efficient in vitro screening model of weight-loss drugs and to conduct preliminary evaluation of the model. Methods Oleic acid was used to induce adipocyte in SD rat primary adipocytes. The model of obesity was established by selecting the positive drug isoproterenol and the functional factor genistein, caffeine and curcumin, Evaluation. Results of the test selected positive test substance can significantly stimulate adipocyte lipolysis, while the negative test substance without lipolysis. Conclusion The model can be used for rapid in vitro screening of diet pills.