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目的 :探讨粉防己碱 (Tet)对离体新西兰白兔阴茎海绵体的松弛作用。 方法 :采用离体新西兰白兔阴茎海绵体肌条张力记录法 ,观察Tet对氯化钾 (KCl)和去氧肾上腺素 (PE)诱导收缩的阴茎海绵体肌条的松弛作用 ;L 硝基精氨酸 (L NNA)和亚甲蓝处理后 ,Tet对PE诱导收缩的阴茎海绵体肌条松弛的作用。 结果 :10 μmol/L及3 0 μmol/L的Tet使KCl诱导的最大收缩反应分别降低为 ( 73 .0± 3 .8) %和 ( 41.5± 3 .4) % ,降低程度与Tet的浓度呈正比 (P <0 .0 1)。Tet对 10 μmol/LPE诱导的肌条收缩具有浓度依赖性松弛作用 ,1、10、3 0、10 0 μmol/L的Tet对PE诱导的阴茎海绵体肌条收缩的松弛效应分别为 ( 6.0± 1.4) %、( 2 1.3± 2 .2 ) %、( 47.4± 3 .3 ) %和 ( 68.1±3 .6) % (P <0 .0 1) ;而L NNA及亚甲蓝对Tet的松弛作用没有影响 (P >0 .0 5 )。 结论 :Tet能浓度依耐性地松弛离体新西兰白兔阴茎海绵体 ,其作用机制可能是通过阻滞钙通道 ,而与一氧化氮 环鸟苷酸 (NO cGMP)通路无关
Objective: To investigate the relaxation effect of tetrandrine (Tet) on isolated corpus cavernosum of New Zealand white rabbits. Methods: The relaxant effect of Tet on contractile muscle strips of penis of KCl and phenylephrine (PE) was observed by the muscle strips tension of corpus cavernosum in vitro in New Zealand white rabbits. Tet (L-threonine) and methylene blue treatment of tetanus to PE-induced contraction of the penis muscle strips relaxation effect. Results: The maximal contractile response induced by KCl was decreased to (73.0 ± 3.8)% and (41.5 ± 3.4%) at 10 μmol / L and 30 μmol / L Tet, respectively. The decrease was related to the concentration of Tet Was proportional (P <0. 01). Tet had a concentration-dependent relaxation effect on muscle contraction induced by 10 μmol / LPE. The relaxation effects of Tet at 1, 10, 30, and 10 μmol / L on PE-induced contractions of penis muscle were (6.0 ± 1.4%, (21.3 ± 2.2%), (47.4 ± 3.3%) and (68.1 ± 3.6%) (P <0.01) Relaxation had no effect (P> 0.05). CONCLUSION: Tet can relax the corpus cavernosum of New Zealand white rabbits in a concentration-dependent manner. Its mechanism may be through the block of calcium channels, but not with the NO cGMP pathway