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目的:研究文拉法新对慢性应激引起的海马CA3区萎缩和凋亡的预防与治疗作用。方法:实验于2004-06/2004-10在中南大学湘雅医学院病理学实验室完成,将SD大鼠随机分成对照组和3个实验组,用强迫性游泳4周的方法对大鼠进行慢性应激处理,断头处死后用石蜡切片苏木精-伊红染色法对海马CA3区组织进行切片染色,在电镜下观察CA3区神经元情况。同时观察各组实验期间的行为。结果:生理盐水对照组大鼠行为均表现正常;应激+生理盐水组大鼠均出现紧张度增加,探究行为受抑制,兴趣丧失或怪异行为;应激+阿米替林组多数出现探究行为抑制、兴趣减少等情况,未见怪异行为;应激+文拉法新组少数出现行为抑制,多数大鼠探究行为正常,未见震颤或怪异行为。生理盐水对照组未出现凋亡,其他各组发生凋亡的情况分别为:应激+生理盐水组:10/16(62%),应激+阿米替林组:6/16(38%),应激+文拉法新组:3/16(19%);经统计学检验表明,应激+阿米替林组出现凋亡的大鼠数和应激+生理盐水组差异没有显著性意义(P>0.05),和应激+文拉法新组差异也没有显著性意义(P>0.05),应激+文拉法新组和应激+生理盐水组差异存在显著性意义(χ2=6.652,P<0.05)。结论:文拉法新对于慢性应激所致的CA3区细胞萎缩与凋亡具有预防和治疗作用,且对探究行为无明显影响。
AIM: To study the preventive and therapeutic effects of venlafaxine on atrophy and apoptosis induced by chronic stress in hippocampal CA3 region. METHODS: The experiment was performed in the Pathology Laboratory of Xiangya School of Medicine, Central South University from June 2004 to October 2004. SD rats were randomly divided into control group and three experimental groups. Rats were subjected to forced swimming for 4 weeks Chronic stress treatment, after decapitation, paraffin sections were processed with hematoxylin-eosin staining staining sections of hippocampal CA3 region, under the electron microscope to observe the neurons in CA3 area. At the same time observe the behavior of each group during the experiment. Results: The behavior of rats in normal saline control group was normal. The rats in stress + saline group showed increased stress, inhibited exploration behavior, loss of interest or strange behavior. Most of the stress + amitriptyline group had exploratory behavior Inhibition, decreased interest and so on, no weird behavior; a small number of stress + venlafaxin group behavior inhibition, most rats explore the normal behavior, no tremor or weird behavior. Apoptosis was not observed in saline control group. The apoptosis in other groups were: stress + saline group: 10/16 (62%), stress + amitriptyline group: 6/16 (38% ), Stress + venlafaxin group: 3/16 (19%); the statistical tests showed that there was no significant difference in the number of rats in stress + amitriptyline group and stress + saline group (P> 0.05), and there was no significant difference between stress + venlafaxin group and stress + venlafaxine group and stress + saline group (P> 0.05) χ2 = 6.652, P <0.05). CONCLUSION: Venlafaxine has a preventive and therapeutic effect on the cell atrophy and apoptosis induced by chronic stress in CA3 area, and has no significant effect on the exploration behavior.