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目的:探讨奥曲肽(生长抑素类似物)对雄激素非依赖性前列腺癌细胞株PC-3的抑制作用及对其血管内皮生长因子(VEGF)表达的影响。方法:分别用浓度为0.1、0.3、1.0、3.0 mg/L奥曲肽对体外培养的人前列腺癌PC 3细胞进行处理,24 h、48 h、72 h后采用MTT法检测细胞生长活性,行Hoechst33258染色,用荧光显微镜观察细胞凋亡的形态学改变;用RT PCR法测定细胞半胱氨酸天门冬氨酸蛋白酶3(Caspase-3)及VEGF的表达。结果:奥曲肽能以剂量与时间依赖性的方式抑制PC-3细胞的生长,促进其凋亡;0.1、0.3、1.0、3.0 mg/L奥曲肽作用PC-3细胞24 h后的存活率分别为(0.971±0.01 6)%、(0.853±0.01 5)%、(0.717±0.031)%、(0.743±0.029)%,作用48 h后的存活率分别为(0.918±0.015)%、(0.835±0.015)%、(0.682±0.018)%、(0.727±0.014)%,作用72 h后的存活率分别为(0.922±0.017)%、(0.841±0.01 3)%、(0.717±0.017)%、(0.739±0.003)%,差别有统计学意义(P<0.01);Caspase-3表达较对照组明显升高;而VEGF则明显降低。结论:奥曲肽能显著抑制PC 3细胞的体外生长,促进其凋亡;VEGF可能是其中的一条途径。
Objective: To investigate the inhibitory effect of octreotide (somatostatin analogue) on androgen-independent prostate cancer cell line PC-3 and its effect on vascular endothelial growth factor (VEGF) expression. Methods: PC3 cells cultured in vitro were treated with 0.1, 0.3, 1.0 and 3.0 mg / L octreotide, respectively. Cell growth activity was detected by MTT assay 24 h, 48 h, 72 h after Hoechst33258 staining The morphological changes of apoptotic cells were observed by fluorescence microscopy. The expression of caspase-3 and VEGF was detected by RT-PCR. Results: Octreotide could inhibit the growth of PC-3 cells in a time-and dose-dependent manner and promote its apoptosis. The survival rates of PC-3 cells treated with 0.1, 0.3, 1.0 and 3.0 mg / L octreotide for 24 h were ( (0.918 ± 0.015)%, (0.835 ± 0.015)%, (0.983 ± 0.01 5)%, (0.717 ± 0.031)%, (0.743 ± 0.029) (0.722 ± 0.018)% and (0.727 ± 0.014)%, respectively. The survival rates after 72 h treatment were (0.922 ± 0.017)%, (0.841 ± 0.01 3)%, 0.003)%, the difference was statistically significant (P <0.01); Caspase-3 expression was significantly higher than the control group; while VEGF was significantly lower. Conclusion: Octreotide can significantly inhibit the growth of PC 3 cells in vitro and promote its apoptosis. VEGF may be one of the pathways.