抗生素是临床上使用最广泛的一类药品，其微生物检定在控制抗生素药品质量中占很重要的地位。但由于抗生素微生物检定操作中影响实验结果的因素较多，为了减少实验误差，保证实验结果的准确性和可靠性，作者结合几年来的工作实践、就培养基质量、试验菌种、缓冲液PH值，培养温 Antibiotics is one of the most widely used drugs in clinical practice. Its microbiology test plays a very important role in controlling the quality of antibiotics. However, due to many factors affecting the experimental results in the operation of the antibiotic microbial test, in order to reduce the experimental error and ensure the accuracy and reliability of the experimental results, the author combined the work practices over the years with regard to the quality of the medium, the test bacteria, the pH of the buffer Value, training temperature