目的　构建赖型钩端螺旋体 0 17株及澳洲型钩端螺旋体 6 0 7株外膜蛋白抗原基因om pL1和内鞭毛抗原基因flaB2 的重组质粒 ,并分别对ompL1及flaB2 基因进行序列分析。方法　通过聚合酶链反应扩增ompL1及flaB2 ,并将其分别克隆到pcDNA3.1/Myc His(+)载体T7启动子下游 ,构建抗原基因表达质粒 ,进行序列测定分析。结果　序列分析显示赖型钩体 0 17株与澳洲型钩体 6 0 7株的ompL1相同碱基 94 9个 (98.85 % ) ,碱基变异 11个 (1.15 % ) ;flaB2 的相同碱基 82 3个 (96 .94 % ) ,碱基变异 2 6个(3.0 6 % ) ,呈很高的保守性。结论　赖型钩体 0 17株与澳洲型钩体 6 0 7株的ompL1及flaB2 分别具有高度同源性 Objective To construct recombinant plasmids of 0 17 strains of Leptospira interrogans and 0 6 strains of Leptospira interrogans and 0 flanders of flagella antigen gene of Leptospira interrogans, and analyze the sequence of ompL1 and flaB2 gene respectively. Methods ompL1 and flaB2 were amplified by polymerase chain reaction (PCR) and cloned into the downstream of the T7 promoter of pcDNA3.1 / Myc His (+) vector respectively. The antigen gene expression plasmids were constructed and sequenced. Results The sequence analysis showed that 9 9 (98.85%) of the same base of ompL1 and 0 (1.15%) of ompL1 were found in 017 strains of Leishmania species and 607 strains of Leptospira interrogans. The same bases of flaB2 (96.94%), 26 base variations (3.06%) were highly conserved. Conclusion ompL1 and flaB2 were highly homologous to the 017 strain of Leptospira interrogans and Australia, respectively