以小鼠脾淋巴细胞为实验材料,采用MTT微量自动比色法判断细胞分裂状况,观察PHA的促淋巴细胞分裂作用时Ca~(2+)的影响。结果发现:加Ca~(2+)的最大刺激组的光密度(OD)值比对照组提高了35.4％,Ca~(2+)的最大刺激浓度为4％葡萄糖酸钙.结果表明Ca~(2+)有增强PHA的有丝分裂原作用,并且存在明显的量—效关系。 The mouse splenic lymphocytes were used as experimental materials, MTT microautomatic colorimetric method was used to determine the cell division, and the effect of Ca ~ (2+) on the proliferation of lymphocytes induced by PHA was observed. The results showed that the optical density (OD) of the group stimulated by Ca 2+ was 35.4% higher than that of the control group, and the maximum stimulation concentration of Ca 2+ was 4% calcium gluconate.The results showed that Ca ~ (2+) enhanced the mitogenic activity of PHA, and there was a significant dose-response relationship.