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自从Feinstone等通过免疫电镜在甲型肝炎(HA)病人粪便中观察到27nm的病毒样颗粒以来,虽经一些研究者证实了对实验动物(狨猴和猩猩)的感染性和用电镜观察到此颗粒的存在。但是,感染动物肝细胞质内病毒样颗粒的抗原特异性一直设有得到直接的证明。本文报导了肝细胞质中27nm病毒样颗粒具有HAAg的特异性及其在肝细胞内超微结构的分布。作者用含有大量27nmHAAg颗粒的人粪便滤液接种狨猴(Saguinus mystar marmoset),当其血清转氨酶开始升高后二周左右宰杀,取其肝组织并作光学显微镜和免疫电镜观察前的常规处理,即固定,冰冻和切片;其次,酶标抗体的制备,使用人工感染猩猩高滴度抗—HAAg免疫血清(免疫粘附血凝法,1:16000),然后
Although Feinstone et al. Have observed the infectivity of experimental animals (marmosets and orangutans) by some investigators since 27 nm of virus-like particles were observed in feces of hepatitis A (HA) patients by immunoelectron microscopy and were observed by electron microscopy The presence of particles. However, the antigen specificity of virus-like particles in the infected animal’s cytoplasm has been consistently demonstrated. This paper reports the specificity of HAAg in 27nm virus-like particles in liver cytoplasm and their distribution in the ultrastructure of hepatocytes. The authors used Saguinus mystar marmoset, a human faecal filtrate containing a large amount of 27 nm HAAg particles, to slaughter it about two weeks after its serum aminotransferase began to rise. The liver tissue was taken for routine treatment before light microscopy and immunoelectron microscopy, Fixed, frozen and sectioned. Secondly, the preparation of enzyme-labeled antibody was performed by artificial infection of orangutan with high titer of anti-HAAg immune serum (Immune Adhesion Hemagglutination, 1: 16000)