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目的:建立组织中延胡索乙素的含量测定方法,并研究元胡止痛方白芷有效部位对延胡索有效部位中延胡索乙素在大鼠组织分布特征的影响。方法:灌胃给药,HPLC-FLD法测定大鼠体内延胡索乙素的组织含量,采用甲醇溶剂沉淀蛋白,上清液氮气吹干,流动相复溶,高效液相荧光色谱法测定大鼠心、肝、脾、肺、肾及脑组织中的延胡索乙素。结果:灌胃给予白芷与延胡索有效部位配伍组1h及2h,延胡索乙素在大鼠组织中体内各组织的含量均有不同程度的增加,其增加程度在心、肝、脑部位更为明显。结论:元胡止痛方中白芷可显著提高延胡索中延胡索乙素在靶器官的含量。
OBJECTIVE: To establish a method for the determination of tetrahydropalmatine in tissues and to study the effect of active fractions of Yuanhu Zhitongfang on the tissue distribution characteristics of Tetrahydropalmatine in the active fractions of Corydalis yanhusuo. Methods: The content of tetrahydropalmatine in rats was determined by HPLC-FLD method. The protein was precipitated by methanol solvent, the supernatant was air-dried with nitrogen, the mobile phase was reconstituted and the content of tetrahydropalmatine was determined by HPLC , Liver, spleen, lung, kidney and brain tissue tetrahydropalmatine. Results: The intragastric administration of Angelica dahurica and ganoderma lucidum in the compatibility group for 1h and 2h showed that the content of tetrahydropalmatine in the tissues of the rats increased in varying degrees, and the degree of increase was more obvious in the heart, liver and brain. Conclusion: Yuanhu Zhitong Fang Angelica can significantly increase the content of tetrahydropalmatine in the target organs.