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目的 :了解鼠疫耶尔森氏菌 16S - 2 3SrRNA基因间区的酶切位点分布情况。方法 :PCR扩增及限制性内切酶分析。结果 :对EV菌及不同来源的 10 3株鼠疫耶尔森氏菌 16S - 2 3SrRNA基因间区进行了扩增 ,选用限制性内切酶TaqⅠ及MspⅠ对扩增产物进行酶切分析 ,结果发现所用鼠疫耶尔森氏菌该间区扩增产物及酶切图谱一致 ;选用TaqⅠ +MspⅠ ,HinfⅠ +MspⅠ对EV菌 16S - 2 3SrRNA基因间区扩增产物进行了双酶切分析 ,作出了TaqⅠ ,MspⅠ ,HinfⅠ在EV菌该间区的酶切位点图。结论 :鼠疫耶尔森氏菌 16S - 2 3SrRNA基因间区相当保守 ;酶切位点分析为对该区进行进一步研究打下了基础
Objective: To understand the distribution of 16S - 2 3SrRNA intergenic region in Yersinia pestis. Methods: PCR amplification and restriction enzyme analysis. Results: The 16S - 23SrRNA intergenic region of Yersinia pestis from 10 3 strains of EV and different origins was amplified. Restriction endonuclease analysis of the amplified product with restriction endonucleases Taq Ⅰ and Msp Ⅰ revealed that The PCR products of Yersinia pestis in this area were consistent with those of the restriction enzyme digestion. TaqⅠ + MspⅠ, HinfⅠ + MspⅠ were used to analyze the amplification products of 16S - 23SrRNA between EV strains. , Msp Ⅰ, Hinf Ⅰ in the EV region between the enzyme site map. CONCLUSIONS: The 16S - 23SrRNA intergenic region of Yersinia pestis is quite conserved; restriction enzyme analysis laid the foundation for further study in this region