此染色法是我们在研究小麦白粉病的过程中和在文献的启发下而探讨出来的。其优点是迅速方便,准确可靠。 一、染液的配制法。按容器大小和需要,分别称取中性红和次甲基蓝,二者分别配成0.2％的水溶液。为了加速次甲基蓝的溶解,可以加温至45℃左右,并加搅拌。待二者充分溶解后将中性红溶液倒于次甲基蓝溶液中。搅拌均匀后即可使用或装在玻璃瓶中备用。 This staining method was explored in our study of wheat powdery mildew and inspired by the literature. Its advantage is rapid and convenient, accurate and reliable. First, the preparation of dye solution. According to the container size and needs, respectively, weighed neutral red and methylene blue, the two were dubbed 0.2% aqueous solution. In order to speed up the dissolution of Methylene Blue, you can heat to about 45 ℃, and add stirring. After the two were fully dissolved, the neutral red solution was poured into methylene blue solution. Stir well and use or in a glass bottle.